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REMOVE THIS IF YOU WANT TO GET RID OF TOP LINKS (RSS, LOGIN, REGISTER | PROFILE) start Log inPurdue University Cytometry Laboratories Welcome, PUCL is the leading cytometry site since 1993. Search this site:Purdue Cytometry Discussion ListNew Jobs Twitter Tweets by @Cytometryman Cytometry Part A- Feed Response to: Comments on the publication Determination of Background, Signal‐to‐Noise, and Dynamic Range of a Flow Cytometer: A Novel Practical Method for Instrument Characterization and Standardization” by Giesecke et al. Automated Data Cleanup for Mass Cytometry Apoptotic Cell Exclusion and Bias‐Free Single‐Cell Selection Are Important Quality Control Requirements for Successful Single‐Cell Sequencing Applications more Cytometry Meetings 2012 Meetings 2011 Meetings 2010 Meetings 2009 Meetings 2008 Meetings 2007 Meetings 2006 Meetings 2005 Meetings 2004 Meetings 2003 Meetings Products Reagents Beads Instruments Services Software & Computer Image Analysis Light Sources Microscopes Optical Software Software Catalog Java Spectra Widgets WinMDI Software FCS Assistant FCS Press FlowJo Demo Confocal Assistant LDATAPP PIC Converter PUCL Software Websites USA - All USA - East USA - Midwest USA - West USA - South Canada UK and Europe Australia and Asia Confocal Organizations General Societies Journals Books Reference in Flow Cytometry Reference in Confocal Microscopy Jobs Positions Available Positions Needed Post-Docs HC Flow References Protocols Cytokine Ref. Chart MAB Ref. Chart Cheap Flights Our Lab Visitors Detailed Directions Local Weather Confocal Microscopy Instrumentation Analysis & Software Capabilities Flow Cytometry Assays Coulter Cytomics FC500 MoFlo XDP High Speed Cell Sorter and Analyzer Bio-Rad BRYTE HS Flow Cytometer Research Current Research Projects Image and 3D Reconstruction Time-lapse Confocal Reflection Imaging Sperm Selection Technologies Microbiology and Flow Cytometry Veterinary Medicine and Flow Cytometry Publications Education Courses Classes BMS 524 - Imaging BMS 631 - Flow Cytometry BMS 633 for BME Students BMS 634 for BME Students Lectures Presenting a Seminar Presenting to General Public CDs & DVDs Video Tutorials Cytometry List Works from PUCL: Seen these covers? They represent work from PUCL Cytometry 51B 21-29, 2003 Cytometry 67A 61-67, 2005 Molecular & Cellular Proteomics 5: 2-13, 2006 Micromech. Microeng. 17 (2007) 1558-1566 DVDs Available: The Purdue group has a new DVD-Booklet package focused on Global Health, aimed at sharing ideas and information about innovative programs in resource-limited countries. We will freely distribute 100,000 copies around the world. Publication date for this product was May 2009. .. This is a high profile project and we encourage individuals and institutions to distribute copies. To do so, please send an email to: disc11@flowcyt.cyto.purdue.edu or go to Global Health DVD A significant amount of sponsorship is available for this project. With 20,000 distribution, this project will be very attractive to sponsors. To inquire about furture opportunities please contact us at: disc11sponsor@flowcyt.cyto.purdue.edu Everest Greeting to Cytometry community from JPR Dr. J. Paul Robinson, PUCL Director has just returned from Nepal having sucessfully summited the world’s tallest mountain at 29,035ft. The effort was closely related to raising awareness of the devastating problems of AIDS in resource-limited countries, with the goal of raising money for Purdue’s nonprofit Cytometry for Life program.C4L is developing low-cost diagnostics to help HIV/AIDS victims in rural Africa receive treatment. You can follow the timeline of the climb his blog . Robinson Lab Current Projects J. Paul Robinson Lab Google Scholar Link to see our publications LASER INDUCED BREAKDOWN SPECTROSCOPY : We have combined Laser Induced Breakdown Spectroscopy (LIBS) with lanthanide-antibody tags to create a unique way to detect pathogens or toxins. We currently designed and built a custom LIBS instrument and we plan to move this to a field deployable hand-held instrument for biological detection. The opportunities extend from bioterrorism, to food pathogens and chemical contaminants. This exciting technology has the opportunity for an innovative approach that incorporates detection, diagnostics and quantification. ELASTIC LIGHT SCATTER : We developed a technology for identification of bacterial pathogens using elastic light scatter (ELS). It uses a laser to interrogate a bacterial colony and the scatter forms a unique pattern onto an imaging sensor. We have developed a number of projects from this technology including new approaches to classification, instrumentation and automation. Here is a link that explanins ELS .... and lots more on this page SINGLE PHOTON SPECTROSCOPY : We are very excited about the opportunities in expanding our current PMT/APD photon measurement technologies to single photon (SP) detection. Of particular interest is the unique expansion of spectral analysis in flow cytometry using single photon detectors . Here is a link to a recent paper on single photon detector design by our collaborator Masaonobu Yamamoto. We have developed our own unique high-speed, low-noise detector and we are moving toward building a 42 ch array detector which we believe will be transformational in biological imaging and the first such detector ever created. The opportunities for integration into multiple detection technologies are endless! Single photon detectors will one day replace many current photon detectors. Here is a link to a recent book we published on this topic NANO-PARTICLE DETECTION : It seems everyone wants to study nano -particles (NP) but few if any technologies can actually achieve measurement of single NPs and let alone characterize them. We have designed a Blu-Ray based technology as a Point Of Care (POC) technology that can analyze particles as small as 20nm . We want to advance this technology by integrating multiple fluorescent detection options, and in the future determine how to sort or separate these particles. There are many applications for this technology – detection of cancer-markers in plasma, monitoring cancer after therapy, monitoring HIV or HBV or potentially any perhaps any viral disease or even plastic particles in the environment. Here is a Youtube link about this project Some of our Software Projects 1. High Throughput Flow Cytometry Analysis Single Cell Analysis New Book from Purdue University Cytometry Laboratories and University of Modena, Italy http://link.springer.com/book/10.1007%2F978-981-10-4499-1 Chapters 1. Microenvironment Cytometry Paul J. Smith, Victoria Griesdoorn, Oscar F. Silvestre, Rachel J. Errington 2. Rare Cells: Focus on Detection and Clinical Relevance Sara De Biasi, Lara Gibellini, Milena Nasi, Marcello Pinti, Andrea Cossarizza 3.E All’ottavo Giorno, Dio Creò La Citometria … and on the 8th Day, God Created Cytometry ” J. Paul Robinson 4. Cytomics of Oxidative Stress: Probes and Problems José-Enrique O’Connor, Guadalupe Herrera, Francisco Sala-de-Oyanguren, Beatriz Jávega, and Alicia Mortinex-Romeroa 5. Flow Cytometry in Multi-center and Longitudinal Studies Anis Larbi 6. Validation—The Key to Translatable Cytometry in the 21st Century Virginia Litwin, Cherie Green, Alessandra Vitaliti 7. Flow Cytometry in Microbiology: The Reason and the Need Cidália Pina-Vaz, Sofia Costa-de-Oliveira, Ana Silva-Dias, Ana Pinto Silva, Rita Teixeira-Santos and Acácio Gonçalves Rodrigues 8. Flow Cytometer Performance Characterization, Standardization, and Control Lili Wang, Robert A. Hoffman 9. Alternative Approaches for Analysis of Complex Data Sets in Flow Cytometry Carmen Gondhalekar 10. Photon Detection: Current Status Masanobu Yamamoto 11. Identification of Small-Molecule Inducers of FOXP3 in Human T Cells Using High-Throughput Flow Cytometry Rob Jepras, Poonam Shah, Metul Patel, Steve Ludbrook, Gregory Wands, Gary Bonhert, Andrew Lake, Scott Davis...